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NMT nicotinamide N-methyltransferase Nicotinamide N-methyltransferase is a key enzyme located in the cytosolic milieu that catalyzes the transfer of methyl group from the co-factor S-(5′-Adenosyl)-L-methionine (SAM) to substrates such as nicotinamide (NCA), pyridine, and related analogs, such as quinoline, isoquinoline, and the aliphatic amine 1,2,3,4 tetrahydroisoquinoline