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After blocking with 5% bovine serum albumin (RMBIO, Missoula, MT, USA)/tris buffered saline with tween 20 (TBST), the membranes were incubated overnight at 4°C with following primary antibodies: rat anti MBP (1:5000, Merck, Darmstadt, Germany), rabbit anti XBP-1s (1:1000, D2C1F, Cell Signaling Technology, Inc., Danvers, MA, USA), extracellular signal-regulated protein Kinase (ERK1/2, 1:20,000, sigma), phospho-p44/42 (1:2,000, Cell Signaling Technology, Inc.), and beta-actin (1:5000, Abcam, Cambridge, MA, USA) antibodies

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