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The homogenate was then centrifuged at 10,000 × g for 15 min at 4°C, the supernatant was collected and the protein concentration was measured using the Bradford method

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A formulation for transdermal delivery of a medicament through the skin of a subject, comprising a therapeutically effective amount of a medicament and a penetrant portion, wherein the penetrant portion comprises: a) phosphatidylcholine (3 - 15% w/w)