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Interaction materials [edit | edit source] These are typical biochemical interactions in nature that have been used extensively in affinity chromatography: โข Enzyme will bind to substrate analogue, inhibitor, and cofactor โข Lectin will bind to polysaccharide, glycoprotein, cell surface receptor, cell โข Antibody will bind to antigen, virus, cell โข Nucleic acid will attach to complementary base sequence, histones, nucleic acid polymerase, and nucleic acid binding protein โข Hormone, vitamin will bind to receptor, carrier protein โข Glutathione will bind to glutathione-S-transferase or GST fusion proteins โข Metal ions will attach to Poly (His) fusion proteins, native proteins with histidine, cysteine and tryptophan residues on their surfaces