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The genes encoding DDB1 , CULLIN4A , RBX1 , DET1 , DDA1 , c-Jun , STK40 , ETS2 , Ube2e family ( Ube2e1 ~ 3 ), Ube2d3, Uba3, UBC12, APPBP1 and NEDD8 were amplified from cDNA by PCR and subcloned into pCAG vectors with different tags, respectively

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