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In some cases, these remedies would require substantial cost and labor and might then present new issues such as regulatory issues

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HepG2 cell culture, treatment and cellular fractionation HepG2 cells were grown in poly-L-lysine coated 75 cm 2 flasks (∼2.0–2.5×10 6 cells/ml) in DMEM medium supplemented with 1% nonessential amino acids, 2 mM glutamine, 10% heat inactivated fetal bovine serum in the presence of 5% CO2–95% air at 37°C as described before [23]