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The published preclinical work (Neelakantan et al., referenced via PMC5826726) reported clean safety at 20 mg/kg subcutaneous in mice over 11 days

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03 · research advantages Selectivity is the reason this compound is used rather than a broader methyltransferase inhibitor: NNMT inhibition can be attributed with reasonable confidence, so a change in NAD+ or SAM levels points to the intended target rather than to an unmapped off-target effect