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However, the cell death caused by ES does not involve the breakdown of the caspase-3, a marker of apoptosis,and not activate caspase-3 (Tsvetkov et al., 2022)

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Briefly, bacterial culture was mixed with 250 μL ethyl acetate/acetic acid (4:1) for 10 seconds by vortexing, and then centrifuged for 5 minutes at 12,000 rpm