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1A Schematic illustration of pathways regulated by NNMT, including the NAD+ biosynthesis salvage pathway starting from NA as a precursor that feeds into energy metabolism, methionine cycle that regulates intracellular SAM concentrations and thus cellular epigenetic modifications and polyamine flux, and clearance of NA by conversion to 1-MNA and excretory products

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The transduced MAP cells were plated on 75 μg/ml hygromycin-7H11 plates and incubated at 37°C for 4-6 weeks to select colonies that had successfully accomplished homologous recombination ( Figure 1A )