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In terms of statistical conclusion validity, the fact that the researchers found significant group differences for several outcomes indicates that statistical conclusion validity was good—but it was not excellent, as the authors themselves noted

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However, while these experiments did not distinguish which KCC may be involved in the stimulatory effects of high doses of the diuretic, inhibition of β-cell KCC2 with highly selective drugs (Kursan et al., 2017) or its transient siRNA-mediated downregulation in islets (Pae and Harper, 2021) resulted in increased insulin secretion in response to glucose