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When an E/M service, other than a preventive care visit, is provided on the same date as a prophylactic immunization, modifier 25 may be appended to the E/M service code

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Most frequently, FCS, which, in addition to the translational diffusion time of the molecules through the small detection volume provides information on the average number of fluorescent molecules in the detection volume, is utilized in detection and identification of single molecules in solution (Eigen and Rigler, 1994