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Thiolate anion formation is activated Activation increases the rate of thiolate anion formation (not the chemical step) Activation of MGST1 by reactive intermediates in vivo (2-3 fold) Diethylmaleate (direct) Acetaminophen P450 P450 CCl4 • CCl3 Reactive quinoneimine In vivo by hydroperoxide GSSG/GSH ratio = 50 at half maximal activation Activation of MGST1 by S-thiolation In vitro by GSSG Sies et al, ABB 322, 288 CAPACITY: 0.2 mM Glutathione transferase in liver + 5 mM GSH = 25 turnovers empties the liver of GSH (e.g

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True IgE-mediated allergy to propofol is exceedingly rare