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Similarly, in a time-resolved Förster resonance energy transfer (TR-FRET)-based proximity assay, M12 alone did not induce proximity between DCAF11 and DDX18, but addition of cellular lysate to the assay buffer enabled M12 to promote the DCAF11–DDX18 interaction (half-maximal effective concentration (EC 50 ) = 1.76 µM)

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(2022), reinforce the conclusion that neuroimmune dysregulation and the loss of glial structural support are central mechanisms in the pathophysiology of depression