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2: Bradford Assay - Biology LibreTexts

After blocking with NcmBlot blocking buffer (P30500, New Cell & Molecular Biotech, Suzhou, China) for 10 min at room temperature, the membranes were incubated with the primary antibody against the following targets: IGF2BP2 (1:2000, 11601-1-AP, Proteintech, Wuhan, China), GSTM5 (1:4000, 14502-1-AP, Proteintech, Wuhan, China), MMP9 (1:2000, ab76003, Abcam, England), MMP13 (1:1000, 18165-1-AP, Proteintech, Wuhan, China), iNOS (1:1000, 18985-1-AP, Proteintech, Wuhan, China), TNF-α (1:1000, 60291-1-Ig, Proteintech, Wuhan, China), METTL3 (1:1000, 15073-1-AP, Proteintech, Wuhan, China), METTL14 (1:1000, 26158-1-AP, Proteintech, Wuhan, China) and GAPDH (1:10000, 60004-1-Ig, Proteintech, Wuhan, China) overnight at 4 °C

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