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Polar metabolites were dried and washed twice in methanol, followed by derivatizing overnight (RT) by methoxymation (20 mg/mL methoxyamine-hydrochloride in pyridine (both Sigma-Aldrich)) and subsequent trimethylsilylation (99:1 BSTFA+TMS (Supelco Analytical)) for more than 1 h, before injection onto the GC-MS