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Sterile technique during reconstitution prevents microbial contamination

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2.1.4 T-bet and Treg cell T-bet is a critical regulator of T regulatory (Treg) cell homeostasis and facilitates the accumulation of T-bet + Treg cells at sites of Th1-mediated inflammation by promoting the expression of the chemokine receptor CXCR3 on Treg cells ( + T-bet + IFN-γ + Tregs are present in the spleen and lungs of infected hosts ( Figure 2D )