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Cells were seeded (4 × 10 3 cells, 40 μL/well) into 384-well culture plates and incubated for 24 h at 37 °C in 5% CO 2

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The limit is lower for CDL holders because there is a lot more responsibility and danger involved in operating large commercial vehicles compared to personal ones, and the penalties are even steeper as a result: The .04 limit applies all the time