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While the body produces glutathione naturally, levels decline with age, stress, poor diet, and environmental toxin exposure, leading many to seek supplementation through various routes including oral supplements, intravenous drips, and intramuscular injections

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This leads to upregulation of pro-inflammatory cytokines and microglial activation in the hippocampus, ultimately suppressing the generation of Bromodeoxyuridine-positive/Doublecortin-positive (BrdU+/DCX+) immature neurons and Bromodeoxyuridine-positive/Neuronal Nuclei-positive (BrdU+/NeuN+) mature neurons