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The study that did not show an overall effect used a DBPC crossover design but not a washout period between the crossover, potentially making it more difficult to observe a significant difference between treatment groups [55]

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Moderate alcohol intake and low MTHFR activity, and the MTHFR677 genotype, (but not he MTHFR1298 genotype) have adverse effects on tHcy, but those effects may be overcome by sufficient folate intake [59,60]