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Leukocytes, platelets and inflammatory cytokines can be perfused into the MOOC devices coated with vascular ECs to investigate the leukocytes/platelets and EC interactions under the inflammatory environment in the process of atherosclerosis and sepsis development (543)(544) Moreover, MOOC devices can also be used to assess endothelial barrier integrity, permeability, and responses to mechanical stimuli or pharmacological agents (541)