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Some other notable examples are the hydroxylation of proline , which is crucial in collagen , and the carboxylation of glutamate to form γ-carboxyglutamate , particularly adept at chelating Ca 2+ , which is important to the cascade of protein factor activation in blood clotting

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Concentration too high — the same total amount of compound delivered in a smaller, more concentrated volume puts more mg into a smaller patch of tissue