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However, there was an increased tendency for migration and invasive behavior

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Cells were resuspended in 100 μ l of binding buffer (10 mM HEPES, 150 mM NaCl, 1 mM MgCl 2 , 5 mM KCl and 1.8 mM CaCl 2 , pH 7.4) then transferred to polystyrene test tubes