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Determining the Change in pH of TSB and 1 × PBS as a Function of GSH Concentration Glutathione powder was weighed and directly dissolved into TSB (pH 7.18) and 1 × PBS (137 mM NaCl, 2.7 mM KCl and 10 mM phosphate, pH 7.41) solution at room temperature under sterile conditions (bio-safety cabinet) to give 10, 20, and 30 mM of GSH

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