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Key Research Areas for Glutathione Intracellular redox status measurement β GSH/GSSG ratio as a primary cellular oxidative stress index Oxidative stress induction and rescue assays β GSH depletion (buthionine sulfoximine, BSO) and repletion studies Glutathione peroxidase (GPx) and glutathione reductase (GR) enzyme activity assays Glutathione S-transferase (GST) detoxification pathway research β xenobiotic and electrophile conjugation studies S-glutathionylation and redox-regulated post-translational modification studies Nrf2/ARE pathway research β transcriptional regulation of GSH synthesis and antioxidant gene expression Mitochondrial GSH pool studies β mtGSH depletion, mitochondrial oxidative damage, and mtDNA protection research Cancer cell biology β GSH-dependent drug resistance, ferroptosis pathway research, GSH-targeted therapeutic strategy studies Neurodegeneration pathway research β GSH depletion in dopaminergic and other neuronal cell models Immune cell function β GSH regulation of T-cell proliferation, cytokine production, and inflammatory signalling Lipid peroxidation and ferroptosis pathway studies β GPx4-GSH axis research GCL/GS enzyme activity and GSH biosynthesis regulation studies in vitro What Do Studies Say About Glutathione
