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On day 8, after 24 h of equilibration in custom media, cells were washed once in PBS, fixed in 4% paraformaldehyde, and permeabilized with 0.5% Triton X-100

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[DOI] [PMC free article] [PubMed] [Google Scholar] 41.Shapiro T.A., Fahey J.W., Dinkova-Kostova A.T., Holtzclaw W.D., Stephenson K.K., Wade K.L., Ye L., Talalay P