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Interaction materials [edit | edit source] These are typical biochemical interactions in nature that have been used extensively in affinity chromatography: • Enzyme will bind to substrate analogue, inhibitor, and cofactor • Lectin will bind to polysaccharide, glycoprotein, cell surface receptor, cell • Antibody will bind to antigen, virus, cell • Nucleic acid will attach to complementary base sequence, histones, nucleic acid polymerase, and nucleic acid binding protein • Hormone, vitamin will bind to receptor, carrier protein • Glutathione will bind to glutathione-S-transferase or GST fusion proteins • Metal ions will attach to Poly (His) fusion proteins, native proteins with histidine, cysteine and tryptophan residues on their surfaces