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Briefly, COS7 cells expressing hCTR or AMY 3 R were seeded at 30,000–40,000 cells per well in 96-well microtiter plates coated with 0.01% poly-l-lysine and grown for 1 day in culture in 100 μL of growth medium, specifically Dulbecco’s modified Eagle’s medium containing 10% fetal calf serum and penicillin (100 IU/mL) and streptomycin (100 μg/mL)

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