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SLT is a newer and more selective procedure that targets individual cells of the trabecular meshwork

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Briefly, 15μL of cDNA from the 10X GEM-RT reaction was input to a PCR reaction (TAP PCR 1) containing a CROPseq-RFP specific forward primer binding 54 bp upstream of the 3’ gRNA sequence (CROPouter) and a reverse primer binding the Illumina Truseq Read 1 (Partial Read 1), producing an amplicon approximately 550-600 bp long covering the gRNA sequence and retaining the 3’ 10X cell barcode and UMI sequences