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Cell culture The CT26, RKO, DLD1, and SW620, 293T, NCM460 cell lines were purchased from the Chinese Academy of Sciences National Collection of Authenticated Cell Cultures (Shanghai, China)

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Moreover, MNP-PDL1 induces M1 polarization of TAMs, with the proportion of CD86+ TAMs cells increasing from 20.6% in the control group to 31.2% and 61.2% in the NP-PDL1 and MNP-PDL1 groups, respectively