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28 Although the chloromethylketone 29 was more active than propiolamide 28 for inhibition of Nek2 in enzymatic assays (IC 50 = 130 nM vs 770 nM), 29 was less active in cells (50% of cells exiting mitosis vs 4% for 28 after treatment with 5 μM of inhibitor for 45 min)