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Discussion CYP4Z1 was originally discovered by Rieger during an investigation aimed at finding genes that are both specific to and highly expressed in breast cancer tissues 7 .Our prior research demonstrated that CYP4Z1 levels was significantly upregulated in breast cancer stem cell tumor microspheres, and the knockdown of CYP4Z1 was shown to diminish the stemness of breast cancer cells by inhibiting PI3K/AKT signaling pathway, thereby reversing the doxorubicin-resistant in these cells 14