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The precursor of most ROS, superoxide anion (2O• 2− ) cannot cross cell membranes, but rapidly reacts to produce hydrogen peroxide (H 2 O 2 ) that can do it, and later on, results in the highly reactive hydroxyl radical (OH•) (Bergamini et al., 2004

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Liver function tests (AST, ALT, GGT, albumin, bilirubin), oxidative stress markers (MDA, SOD, catalase), and nutritional status (cysteine, methionine, B vitamins) provide context