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[DOI] [PMC free article] [PubMed] [Google Scholar] Peng Y., Chen L., Ye S., Kang Y., Liu J., Zeng S., et al

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The coupling reaction was initiated by incubation at 25 °C for 1 h and was followed by the addition of Apyrase (New England BioLab) to catalyze the hydrolysis of GDP overnight at 4 °C