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For the GHK-Cu pretreatment groups, mice were injected intraperitoneally with GHK-Cu (1 or 10 μg/g, every 24 h, for 3 days) dissolved in saline before intratracheal administration of LPS

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Others utilized in vitro exposure of gametes to peptide-supplemented media and found similar benefits, which aid in decoupling direct gamete effects from whole-animal systemic changes