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This work also was supported by the Beijing Chao Enxiang Traditional Chinese Medicine Heritage and Development Foundation 2023 “Towards a New Horizon” Research Project (Project No.2023CX03)

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This chapter provides a comprehensive overview of the key approaches and technologies used to investigate interorgan interactions, including traditional anatomical studies, cutting-edge molecular imaging techniques, disease-specific models, animal experiments, organoid systems, molecular-level analyses, and artificial intelligence-driven methodologies